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Image Search Results
Journal: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
Article Title: Improved multidetector asymmetrical-flow field-flow fractionation method for particle sizing and concentration measurements of lipid-based nanocarriers for RNA delivery.
doi: 10.1016/j.ejpb.2021.03.004
Figure Lengend Snippet: Fig. 2. MD-AF4 results using the LC. A) UV fractograms of LNP1-RNA overlaid with Rg values, measured by Lab1 using the LC with two different XF profiles (linear and exponential) presented in figure C, showing (i) a large void peak (orange arrow), (ii) the main peak associated with NPs (violet arrow) and (iii) a large, retained peak (light blue arrow). ; B) UV fractograms of the same sample overlaid with Rg values, measured by Lab2 using the LC with the linearly decreasing XF profile of Figure C but with focusing times of 4, 7 and 9 min. C) XF profiles applied: exponential (black) and linear (red) decreases of the XF. D) Void peak height as % of the main peak for varying focus times with linear XF profile, Lab2. E) Retained peak height as % of the main peak for linear and exponential XF profiles, Lab1.
Article Snippet: Two widely available commercial platforms were used for this study: in Lab1, an AF2000 Multiflow FFF (Postnova Analytics) and in Lab2 an
Techniques:
Journal: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
Article Title: Improved multidetector asymmetrical-flow field-flow fractionation method for particle sizing and concentration measurements of lipid-based nanocarriers for RNA delivery.
doi: 10.1016/j.ejpb.2021.03.004
Figure Lengend Snippet: Fig. 3. Comparison of the FI and LC AF4 methods. Fractograms obtained for LNP1-RNA by applying a linearly decreasing XF profile for Lab1 (black) and Lab 2 (red) using (A) the LC and (B) the FI channel. Light scattering intensity and UV–Vis absorption are reported on the left and right panels, respectively, together with the Rg profile estimated by Berry model. C) Summary of the results averaged over 3 measurements. Values in parentheses represent the standard deviation of the mean.
Article Snippet: Two widely available commercial platforms were used for this study: in Lab1, an AF2000 Multiflow FFF (Postnova Analytics) and in Lab2 an
Techniques: Comparison, Standard Deviation
Journal: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
Article Title: Improved multidetector asymmetrical-flow field-flow fractionation method for particle sizing and concentration measurements of lipid-based nanocarriers for RNA delivery.
doi: 10.1016/j.ejpb.2021.03.004
Figure Lengend Snippet: Fig. 7. LNP1-RNA particle morphology by MD-AF4. A) Rg and Rh profiles of LNP1-RNA measured by online DLS and online MALS; B) calculated Rg/Rh ratio across the peak.
Article Snippet: Two widely available commercial platforms were used for this study: in Lab1, an AF2000 Multiflow FFF (Postnova Analytics) and in Lab2 an
Techniques: